Method for Obtaining Cleaned Putrefactive Anaerobe 3679 Spores

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Method for Obtaining Cleaned Putrefactive Anaerobe 3679 Spores.

Sporulating clostridial suspensions tend to recycle through subsequent phases such as germination, outgrowth, and further multiplication. The result is a mixture of vegetative cells and of spores at various developmental stages. Recently, activated-culture techniques have been used to achieve almost complete sporulation in semisynchronized populations of several clostridia (Collier, 1). 10, in ...

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Production and cleaning of spores of putrefactive anaerobe 3679.

Putrefactive Anaerobe strain 3679 (PA 3679) is of interest to the food microbiologist because it has been responsible for the microbial spoilage of certain types of thermally processed foods. It is also of interest because the spores of this organism have a high degree of heat resistance and their thermal death rate is frequently used in the calculation of thermal processes for low acid canned ...

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Sequence of events during germination of putrefactive anaerobe 3679 spores.

The sequence of changes during germination of putrefactive anaerobe 3679h spores was studied under aerobic conditions in a solution containing l-alanine and sodium pyrophosphate. Evidence that specific changes occurred in two distinct regions of the spore is given by data on several criteria that were used to measure germination. During the initial stage of germination, the absorbancy decreased...

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Nutritional requirements for germination, outgrowth, and vegetative growth of putrefactive anaerobe 3679 in a chemically defined medium.

Fujioka, Roger S. (University of Hawaii, Honolulu), and Hilmer A. Frank. Nutritional requirements for germination, outgrowth, and vegetative growth of putrefactive anaerobe 3679 in a chemically defined medium. J. Bacteriol. 92:1515-1520. 1966.-A chemically defined medium was used to study the nutritional requirements for germination, outgrowth, and vegetative growth of putrefactive anaerobe 367...

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Growth inhibition of putrefactive anaerobe 3679 caused by stringent-type response induced by protonophoric activity of sorbic acid.

The inhibitory effects of potassium sorbate on the bioenergetics, phenylalanine uptake, protein synthesis, and certain aspects of cell regulation were examined in putrefactive anaerobe 3679. Undissociated sorbic acid appeared to act as a protonophore by lowering the intracellular pH and dissipating the proton motive force of the membrane. Sorbate inhibited the uptake of phenylalanine, decreased...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1965

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.89.3.929-930.1965